| Wavelengths and filters | HE-800: filter selection 340–850 nm; 8-position filter wheel with 405, 450 and 620 nm installed; half-bandwidth 8 ± 2 nm; optional filters 340, 375, 414, 492, 520, 530, 540, 550, 560, 570, 593, 596, 630, 650, 690, 740, 750 and 820 nm, ordered separately; quartz-halogen lamp. HE-201: 405, 450, 492 and 630 nm plus 4 free filter positions; wavelength accuracy < 2 nm; LED light source, > 100,000 hours. HE-202: 405, 450, 492 and 630 nm, 4 more filters optional; tungsten halogen lamp 12 V / 20 W, > 2,000 hours | List the wavelengths your kits use, including any reference wavelength, and confirm which filters are fitted and which are quoted as extras. |
|---|
| Plate formats and reading speed | HE-800: 96-well and 384-well plates, maximum plate height 15.3 mm; 96-well plate 7 s (fast mode) or 13 s (normal mode); 384-well plate 13 s (fast) or 34 s (normal). HE-201, HE-202: 96-well plates with U, V or flat bottom; 8-channel optical system; 5 s single wavelength, 10 s dual wavelength; end-point, kinetic and multi-wave analysis | If you use 384-well or tall plates, choose the HE-800 and check your plate height against the 15.3 mm limit. |
|---|
| Range, accuracy and linearity | HE-800: read-out range 0–6 Abs; resolution 0.001 Abs; linearity 0–3 Abs ± 2% (96-well) and 0–2.5 Abs ± 2% (384-well) at 405 nm; accuracy ± 1% (0–3 Abs) or ± 0.003 Abs, whichever is greater, at 405 nm; precision CV ≤ 0.2% (0.3–3 Abs) at 405 nm. HE-201: reading range 0.000–4.000 Abs, measurement range 0.000–3.000 Abs; well coincidence ≤ 0.015 A. HE-202: measurement range 0–3.00 Abs; resolution 0.001 Abs displayed, 0.0001 Abs internal. HE-201, HE-202: linearity r > 0.999; repeatability < 0.3%; stability < ± 0.005 Abs / 10 min | Ask for the test conditions behind each figure so you can compare the HE-800 with the HE-201 and HE-202 on the same basis. |
|---|
| Shaking and incubation | HE-800: linear shaking with slow, medium and fast modes; optional incubator from ambient +4 °C up to 50 °C. HE-201, HE-202: plate shaking with adjustable speed and time; no incubator listed | If your protocols need incubation in the reader, state it so the HE-800 incubator option is included in the quotation. |
|---|
| Display, printing, interfaces and data | HE-800: 7-inch colour capacitive touch screen; three shortcut keys for saved protocols; software for plate layout, calculation, interpretation and quality control; USB port for data transfer and USB port for printer; external HP PCL5, PCL5c or PCL5e printer; start-up self-diagnosis; 400 × 290 × 220 mm; 100–240 V, 50/60 Hz via adapter. HE-201: built-in computer, 10.4-inch colour touch LCD; 44 × 37 × 24 cm, 13 kg. HE-202: external computer and LCD screen; PSTN and PC connection; 43 × 34 × 16 cm, 9 kg. HE-201, HE-202: open system; storage for 1,000 test items and 1,000,000 results; Westgard multi-rule QC alerts; self-check and calibration; external inkjet printer; RS-232 and USB; 220 V AC or 110 V AC ± 15%, 50–60 Hz, 100 W; operation at 5–40 °C, humidity ≤ 85% | Ask whether a printer and, for the HE-202, a computer and screen are included, how results are exported, and state your mains voltage and plug type. |
|---|